Part:BBa_K1114510:Design

Level 1 MoClo device that expresses YFP under pTetR

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

pR40B2Y_FG This is a Level 1 MoClo device that expresses YFP under the repressible promoter pTetR. It contains the Level 0 parts R0040_FB, BCD2_BC, E0030_CD, and B0015_DG within the DVL1_EF backbone. In the given sequence the internal fusion sites are incorrectly duplicated. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. See Level 0 pages for further information.

Source

Construct of MoClo Level 0 parts derived from iGEM Distribution Kits